Specimen field number: B320 RY-NW-HL 3

Habitat: Low intertidal zone, growing on rock
Abundance: Very common
Nearby species: Ulva linza and ulva fenestrata
Reproduction state: unknown, currently in 1N gametophyte phase

Description:
Bright green in colour. Comprised of small, thin matted ropes. Hooks are present in low concentrations.

Field ID: BIOL320 AC-RZ-AL-JY 7
Physical Description: Papilla are visible on the surface with each possessing either no or a single cystocarp. Large thick, dark red blades.
Collection Date/Time: 3:32 PM (PST) - March 30, 2024
Location & attachment: Mid-intertidal. Attached to a rock
Surrounding Species: Acrosiphonia sp., Ulva intestinalis, Pyropia, sp.

Field ID: BIOL320 AC-RZ-AL-JY 2
Physical Description: Main central axis with finely branched filaments. Polysiphonious thalli construction visible under the microscope. Presence of tetrasporangia indicate that this individual is reproductive.
Collection Date/Time: 4:21 PM (PST) - March 30, 2024.
Location & attachment: Mid-intertidal. Drifting.
Surrounding Species: Ulva sp., Pyropia sp., Saccharina latissima

Field ID: BIOL320 AC-RZ-AL-JY 1
Physical Description: Red. It has veins that rejoin to form a network. Tetrasporangia (dark spots) indicate that this individual is reproductive.
Collection Date/Time: 3:36 PM (PST) - Mar 30, 2024
Location & attachment: Mid-intertidal. Drifting
Surrounding Species: Ulva sp., Pyropia sp.

Field ID: B320-JF-LQ-KZ-16
Growing on: drift
Tidal zone: collected from intertidal but was drift
Time collected: 3:57pm
How common: Only one found, scarce
Nearby species: Ulva, Acrosiphonia, Saccharina, Barnacles, Chondracanthus
Specimen reproductive: no reproduction observed
Identification: Branch tips pointed, plant regularly alternately branched. There are many short branches shown in photo 1. Reddish brown, tough and cartilaginous. Plant compressed to flattened, not bladelike. Branches terete or occasionally slightly compressed. Final photo is a dried specimen.

Field ID: B320-JF-LQ-KZ-6
Growing on: attached to small pebble
Tidal zone: mid intertidal
Time collected: March 30, 2024 at 3:17pm
Abundance: Not abundant, only a few specimens found
Nearby species: Hildenbrandia, barnacles
Specimen reproductive: cystocarps present (photo 1)
Distinguishing characteristics: Thick, papillate blade (photos 1-3), stipe with bladelets (photos 1, 5, 6). Final photo is a dried specimen.

Specimen Field ID: B230-JF-LQ-KZ-1
Growing on: Big boulder
Tidal zone: Mid to low intertidal
Time collected: 3:43 pm
How common: Abundant in nearby clusters.
Nearby species: Ulva, Fucus, barnacles, Oysters
Specimen reproductive (tetrasporophytes).
Identifiable through microscopy (polysiphonous), branching in one plane; lies flat.

Specimen field ID: B320-RH-SD-4
Growing on: rock
Tidal zone: mid
Time collected: 15:00
Common
Features: Microscopic observation - Filamentous with lateral branches to help ID species, no hooks present in filaments, and therefore species is arcta.

Specimen field ID #: B320 HT-GC-RH 4
Growing on: Unattached rock
Tidal zone: Low
Time collected: 3:36 pm
How common? Not very abundant
Nearby species? Acrosiphonia, Mastocarpus, Saccharina
Specimen reproductive? Yes
What features help identify this to genus and/or species? Thick blades frequently papillate; low intertidal; Stipe with 1 or 2 bladelets. 1-3 cystocarps per papilla.

B320 HT-GC-RH 2
Growing on: nothing, was drift
Tidal zone: mid to high intertidal, found drift
Time collected: 3:20 PM
Commonality: not very abundant, a couple other drift specimens scattered around the beach.
Nearby species: Acrosiphonia, Ulva.
Reproductivity: non-reproductive
Identifiable features: root-like holdfast, ruffling on blade

Specimen field ID #: B320 HT-GC-RH 5
Growing on: Rock
Tidal zone: Low, close to waterline
Time collected: 4:18 pm
How common? Fairly abundant
Nearby species? Ulva, Acrosiphonia, Wildemania
Specimen reproductive? Yes. Cystocarps present, indicating that this individual is a female gametophyte.
What features help identify this to genus and/or species? Radial branching; pericentral and axial cells of equal length; more than 4 pericentral cells; ~10cm tall.

Specimen field ID #: B320 HT-GC-RH 3
Growing on: Rock
Tidal zone: Mid-low
Time collected: 3:32 PM
How common?: Extremely abundant
Nearby species?: Ulva linza
Specimen reproductive?: No
What features help identify this to genus and/or species? Branches developing laterally from cells, of the same or larger diameter than axes bearing them. No hooked branchlets observed.

Specimen field ID#: B320 YZ-WW 8
Growing on: unattached, on the rocks
Tidal zone: mid intertidal zone
Time collected: 2024/03/30 3:31 PM
How common: very rare, only one found
Nearby species: Acrosiphonia arcta
Specimen reproductive: Yes
Features to help identify: umbrella shaped, short stipe in the middle of a circular blade. Dense, fine, and inconspicuous blade veins radiating from centre.

Specimen Field ID: #: BIOL320 HB-JW-KC-MB 12
Growing on: Chondracanthus (epiphyte)
Nearby Species: Pyropia, Ulva fenestra, Acrosiphonia
Tidal zone: Low intertidal
Time collected: 2024/03/30 3:25 PM
SCARCE
Identifying features: red, epiphytic (growing on Chondracanthus), terminal branches pinched like crab claws

Specimen Field ID: #: B320 HB-JW-KC-MB 3
Growing on: Rock
Nearby Species: Acrosiphonia, Ulva Frenestrata
Tidal zone: Low to upper intertidal
Time collected: 2024/03/30 3:49 PM
SCARCE
Identifying features: small, dark brown, small discoid (round) holdfast, not a root-like holdfast. The stipe is very short and thin, not a prominent thick stipe (in a kelp).

Specimen Field ID: #: BIOL320 HB-JW-KC-MB 10
Growing on: sand/rocks
Nearby Species: Ulva Fenestrata, Acrosiphonia, Ulva linza, pyropia
Tidal zone: waterline (lowest tide)
Time collected: 2024/03/30 4:00 PM
Relatively LOW abundance
Reproduction: tetrasporangium
Identifying features: red, polysiphonous, lays flat (branching in one plane, pinnate)

Specimen Field ID: #: B320 HB-JW-KC-MB 9
Growing on: rock
Nearby Species: pyropia, Ulva fenestra, Mastocarpus, Petalonia
Tidal zone: Low to High
Time collected: 2024/03/30 3:39 PM
Abundant/scarce/some others/if drift, others nearby: Very abundant
Identifying features: Green algae, threadlike filamentous, multinucleate, no visible hooked cells

Specimen field ID #: B320-JC-AS-GL-VP-4
Growing on: Drift at the waterline
Tidal zone: Mid-intertidal zone
Time collected: 4:11pm
How common: Scarce
Nearby species: Ulva, red algae blades, filamentous green algae
No visible reproductive structures
Features used to identify: Red blade with visible veins that join and branch to form a crisscrossing network.

iNat description: Mid Intertidal, Desmarestia herbacea and Nereocystis leutkeana.
Specimen field ID #: B320-JC-AS-GL-VP-2
Growing on: Clam shell
Tidal zone: Mid intertidal
Time collected: 3:35 pm
How common: Uncommon
Nearby species: On the same shell with Nereocystis leutkeana young sporophyte and Desmarestia. Ulva spp. (common), Petalonia spp. (common), Pyropia spp. (common), Saccharina latissima
Reproductive structures: none identified
Features used to identify:
Opposite branching with cylindrical lower axes
Stringly brown algae with a large stringy thallus

Specimen field ID #: B320-JC-AS-GL-VP-1
Growing on: Shell at waterline
Tidal zone: Mid-intertidal zone
Time collected: 3:28pm
How common: Scarce
Nearby species: The specimen was growing on the same shell as other brown algae. Species near the shell include Ulva, Pyropia, and filamentous green algae
No visible reproductive features
Features used to identify: Brown thallus with ligulate branches that are more than 1 centimeter wide

B320-EH-JL-RA-WJ 17

Found growing on rock in water at low tide (4:20pm).

Not common. Saccarina latissima and Wildemania amplissima found growing nearby.

No evidence of reproduction.

Short branches of this red algae are pinched in where they attach to stem.

Specimen field ID: B320-EH-JL-RA-WJ-16

Growing on shell fragment.
Subtidal. Submerged in water near water line.
Time collected: 4:17pm
Uncommon.
Nearby species: Lots of ulva floating in the water and acrosiphonia on the rocks nearby.
Specimen not determined to be reproductive.

Identified based on long stipe with globose float and terminal blades.

Specimen Field ID: B320-EH-JL-RA-WJ-13

Growing on the lower side of a rock (epilithic).
A couple meters from the waterline (low intertidal).
Time collected: 4:00pm
Uncommon.
Nearby species: lots of fucus growing on upper portions of the rock. Detached ulva found washed up on rock.
Specimen not determined to be reproductive.

Identified based on thin thallus, brown colour, lack of midrib, very small disc shaped holdfast and epilithic location.

B320-EH-JL-RA-WJ 9
Found attached to a rock with a red algae attached
Low intertidal zone
Time collected: 3:43 PM
Abundant
Nearby species: Ulva, Acrosiphonia, Saccharina latissima
Not reproductive
Features to help identify: Ribs five; blade bullate (blistering)

Species field ID#: B320-EH-JL-RA-WJ-7
Determined by flattened blades with non-dichotomous pennate branching to several orders, with main axes larger than 4mm.

Growing On: epilithic
Tidal Zone: in water
How Common: rare
Nearby Species: Acrosiphonia arcta and Wildemania amplissima
Specimen reproductive: not reproductive

Red alga (Phylum Rhodophyta, Class Florideophyceae), specimen B320-TBS-7
Identifying features: Blue iridescence, complex thallus with filamentous medulla, stretchy and slightly thick red blade
Growing on: Growing on large rock
Intertidal zone: Low intertidal
Time collected: March 30 2024, 4:22PM
Commonality: Fairly common
Nearby species: Ulva fenestrata, Ulva intestinalis, Odonthalia sp.
Reproduction: No

Green alga (Phylum Chlorophyta, Class Ulvophyceae), specimen B320-TBS-5

Identifying features:

• Gametophyte (macroscopic filamentous, haploid phase)
• branched with individual cells (not coenocytic)
• green pigment throughout
• branches coming out laterally (from sides of other cells rather than tips) identifies to genus
• lack of hooked branchlets identifies to species (A. acrta rather than A. coalita)

Growing on: Rock
Intertidal zone: All throughout intertidal (from high to low)
Time collected: March 30 2024, 3:40PM
Commonality: Many
Nearby species: Ulva fenestrata, Pyropia sp.
Reproduction: Unknown

Acrosiphonia arcta
Specimen ID#: B320-AH-CJ-JB-JT 1
Growing on: Rocks
Tidal Zone: Low intertidal
Time Collected: 3:56 PM
How common: Very common
Nearby Species: Ulva spp.
Specimen reproductions: Gametophyte
What features help identify this to genus and/or species: The filaments have no hooks

Specimen field ID: “B320-AH-CJ-JB-JT 3
Growing on: Drift
Tidal Zone: Subtidal
Time Collected: 3:53 PM
How Common: common
Nearby Species: unknown
Specimen reproductive: young sporophyte, no sori
What features help identify this to genus and/or species: divided blade, pneumatocyst, holdfast, colour (brown)

Specimen field ID: B320 AH-CJ-JB-JT 6
Growing on: Rocks/attached to a substrate
Tidal Zone: Low-mid intertidal
Time Collected: 4:06 PM
How Common: Somewhat common
Nearby Species: Mastocarpus,
Specimen reproductive: Probably tetrasporophyte
What features help identify this to genus and/or species: Red colour, veins cross

Specimen field ID: “B320-AH-CJ-JB-JT 2
Growing on: Drift
Tidal Zone: Subtidal
Time Collected: 4:38 PM
How Common: somewhat common
Nearby Species: Unknown
Specimen reproductive: Young sporophyte, no sori
What features help identify this to genus and/or species: Single undivided blade with 5 parallel ribs running it’s length, colour

Specimen field ID: B320 AH-CJ-JB-JT 7
Growing on: rocks
Tidal Zone: splash-zone
Time Collected: 4:20 PM
How Common: very common
Nearby Species: None
Specimen reproductive:
What features help identify this to genus and/or species: Microscopy shows square-like cells, location, green colour, small

Specimen ID: B320 DN-CT-SZ-AD 7
Growing on: epilithic
tidal zone: mid intertidal
Time collected: 1500-1600
how common: common
nearby spp:
specimen reproductive: Reproductive (observed tetrasporangia)
red filamentous. gland cells terminal on determinate branches. whorled branching.

Field ID #: B320-KB-DPW-6
Growing on rock in the lower intertidal.
Collected around 4:00pm
Found associated with Ulva spp. Acrosiphinia spp., and Pyropia spp.
No visible reproduction
Polysiphonious constriction with irregular/radial branching

B320 - KB - DPW - 5
Found on the beach - in drift
Found in the mid to upper intertidal
Collected at 3:38pm
The only Savoiea nearby
Surrounded by Ulva spp., and Acrosiphonia sp.
Contains tetraspores - reproductive tetrasporophyte
Polysiphonious construction, feathery thallus lays flat

Specimen field ID #: B320-JC-XY-SK-2
Growing on rock
Low intertidal zone
Time collected: 3:34pm
Abundant
Nearby: Petalonia, Acrosiphonia, Wildemania
Specimen non-reproductive
Distromatic (2 cell layers thick), flat blade with holes

Specimen field ID #: B320-JC-XY-SK-1
Growing on rock
Low intertidal zone
Time collected: 3:34
Abundant
Nearby: Ulva, Acrosiphonia, Wildemania
Non-reproductive
Identifying features: Medullary cells 4-5x bigger than surface cells, surface cells with 1 plastid and 1-2 pyrenoids

On boulder, low intertidal. 0.7m tide and this was very close to the water line at low tide.
Kelp, distinct single midrib through blade. No sporophylls (I’ve never seen sporophylls at this site, it’s weird. Are they all on subtidal specimens? How does this population propagate?!).

Empress figurehead, Stanley Park

Drift specimen, very common
Dark reddish brown, almost black
Irregular branching. Branches pinch in where they meet the main axis but the branches are not nearly as turgid and noodle-like as Sarcodiotheca (see last photo).

Drift specimen, very common.
Branches “pinch in” where they join the main axis, are quite thick and terete. Much more turgid and noodle-like compared to the similar red seaweed Sarcodiotheca (see last photo).

Specimen ID: B320 KD-AS-MT-8
A macroscopic, branched green filament that is not coenocytic, with hooked branches found south of Alaska.
Attached on rock.
Nearby species: red, green, brown algae; spread all across the beach
Tidal zone: low/mid intertidal
Very abundant
Reproductive: No

specimen B320-TBS-1

Identifying features: Flattened, highly branched blades, with further branching also seen on the lateral branches; conspicuous midrib; fringed with pigmented hairs; and a narrow main axis
Growing on: Was found detached from substrate
Intertidal zone: Low intertidal
Time collected: March 30 2024, 3:46PM
Commonality: Rare
Nearby species: None, was found in the water near the shore
Life cycle Stage: Sporophyte

B320-EH-JL-RA-WJ-11
Drift in water
Low intertidal
Collected at 3:53 PM
Rare
Nearby species: Ulva, Acrosiphonia, Saccharina latissima
Not reproductive
Features to help identify: Plant terete with narrowing at base of each branch, found subtidal

Field ID: BIOL320 AC-RZ-AL-JY 3
Physical Description: Dark red. Branching is alternate and distichous.
Collection Date/Time: 4:12 PM (PST) - March 30, 2024
Location & attachment: Low intertidal. Drifting.
Surrounding Species: Ulva sp., Pyropia, sp., Chondracanthus exasperatus.

Specimen field ID #: B320-JC-XY-SK-5
Growing on boulder
Low to mid intertidal zone
Time collected: 3:50pm
Some others
Nearby: Acrosiphonia, Ulva fenestrata
Cystocarps on female gametophyte
Identifying features: Whorled branching, gland cells terminal

Habitat: Low intertidal, growing on rock
Abundance: Very Common
Nearby species: Pyropia sp., Acrosiphonia sp., Ulva fenestrata
Life history: unknown

Description:
Small, long, bright green blades growing in clusters. The thin blades are tubular at base and have ruffled edges.

Brown seaweed with a branching blade wider than 1cm with visible midrib.
Collected 2024/03/30 4:11 PM
Found in the low intertidal, drift
surrounding species: Acrosiphonia, Ulva, Pyropia. Single drift individual, probably washed up from lower height

Red alga (Phylum Rhodophyta, Class Florideophyceae), specimen B320-TBS-8
Identifying features: Red blade with noticeable criss-crossing veins. Veins join together as well as branch.
Growing on: Found washed up in the sand. Unsure what it was growing on.
Intertidal zone: Mid-high intertidal
Time collected: March 30 2024, 3:08PM
Commonality: Rare. We only found 1 specimen
Nearby species: None
Reproduction: Reproductive. Had tetrasporangia.

Collected at 3:50PM, this species was located at the waterline at the mid intertidal zone loosely situated on top of a rock. Ulva and other abundance of green algae were found nearby. There are whorled branching patterns with each whorl consisting of three to four (sometimes two) determinate branches of unequal length. Gland cells are also found at the terminal of the cells of the determinate branches. It was the only Hollenbergia subulata present in this area, appearing to be scarce. Reproductive structures, tetraspores, are found on the branches of this species.

Specimen field ID #: B320 AL-MK-TL-WD 6
Growing on: Drift
Tidal zone: Low intertidal
Time collected: 3:37
How common?: Not common
Nearby species?: Other brown alga
Specimen reproductive?: No
Which features help identify this to genus and/or species?: Opposite branching, doesn't lie flat, cylindrical

Specimen field ID #: BIOL 320 JF-LQ-KZ
Growing on: Rock
Tidal zone: Middle intertidal zone
Time collected: 16:41
How common: Only one found in the area
Nearby Species: Ulva, Acrosiphonia and Cryptosiphonia
Specimen Reproductive?: No evidence
What features help identify this to genus and or specimen?: Translucent gland cells are subterminal on whorl branches, three to four determinate branches

B320-EH-JL-RA-WJ 8

Found as drift in water at low tide (3:41pm).

Not common. Wildemania amplissima and Saccharina latissima growing nearby.

No signs of reproduction.

Branching generally opposite, lower axes cylindrical.

B320-EH-JL-RA-WJ 15

Found in water at low tide (4:15).

Not common.

Saccarina latissima found nearby.

No evidence of reproduction.

Branching opposite, several orders of narrow lingulate branches.

Specimen Field ID: B320-RH-SD-2
Growing on rock.
Mid intertidal zone.
Collected at 3 pm.
Common.
Reproductive tetrasporangia present. (Dark spheres seen in second photo)
Identifiable by the terminal gland cells observed on the determinate branches.

Specimen field ID #: B320-JC-AS-GL-VP-6
Growing on: Drift, found at shore line
Tidal zone: Mid-intertidal, found at water-line adrift
Time collected: March 30, 4:22 pm
How common: Uncommon
Nearby species: Ulva spp. (common), Petalonia spp. (common), Pyropia spp. (common),
Reproductive structures: Cystocarps present
Features used to identify: Cystocarps present ornamented with a spine and are evenly distributed throughout the blade, leathery texture, one or two cystocarps per papilla (primarily one)

Field ID: B320JF-LQ-KZ-8
Growing on: A boulder
Tidal zone: Mid to lower intertidal
Time collected: March 30th at 3:35pm from Stanley Park, B.C.
Abundance: Very abundant
Nearby species: Fucus distichus, oysters, barnacles, Pyropia, Ulva
Specimen reproductive: no evidence of reproduction
Distinguishing characteristics: Dense, tufted plant (photos 4 and 5). Branchlets are not curved or hooked (photos 1, 2, 3). Final photo is a dried specimen.

Field ID: B320-JF-LQ-KZ-2
Growing on: Firmly attached to boulder
Tidal zone: Mid intertidal zone
Time collected: 5:36pm on Sunday, March 31st from Stanley Park, B.C.
How common: Common, quite a few patches found
Nearby species: Ulva, acrosiphonia, pyropia, barnacles
Specimen reproductive: no evidence of reproduction
Distinguishing features: Plant erect. Plant pyramidal in shape (photos 1 and 2), branch lengths decrease uniformly from base to apex. Final photo is a dried specimen.